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This training guide brings together information from existing training materials that aim to reduce the stigma and discrimination directed at key populations by health care workers. It also orients health care workers to the best clinical standards of care for key populations. The guide has been piloted in several different countries, and feedback from ministries of health, trainers, health care workers, peer educators, and LINKAGES staff has been incorporated into the guide. IntraHealth is a partner on LINKAGES and several staff contributed to the guide.